ABSTRACT
Background: Streptococcus gallolyticus (SG) may alter the colon microenvironment through its metabolites, and transforming the human body into a high-risk state prone to tumor formation. Aims: To investigate the effect and mechanism of Streptococcus gallolyticus metabolites (SGM) on growth of colon cancer cells. Methods: Human colon cancer cell line SW620 was conventionally cultured, and were divided into blank group (group A), healthy intestinal flora group (group B), Bifidobacterium metabolites group (group C), SGM group (group D) and Bifidobacterium metabolites + SGM group (group E). CCK-8 test was used to detect the proliferation of tumor cell. Cell migration ability was detected by scratch test. The protein expression of β-catenin was determined by Western blotting. The mRNA expression of c-myc was measured by real time PCR. Results: Compared with group A, group B, group C, cell proliferation was significantly increased (P0.05). Conclusions: SG can enhance the proliferation and migration ability of colon cancer cells through its metabolites, which may be through the activating of Wnt/β-catenin signaling pathway. Moreover, Bifidobacterium metabolites cannot inhibit the enhancement of proliferation and migration ability of colon cancer cells by the SGM.
ABSTRACT
Objective To investigate the effect of narrative education on emotional and clinical ability of baccalaureate nursing students practicing in ICU. Methods A total of 42 baccalaureate nursing students practicing in ICU between January 2016 and September 2016 were treated as the control group and received traditional teaching, their counterparts practicing in ICU between October 2016 and June 2017 were served as the observation group (41 cases) and subject to narrative education. The Self-rating Anxiety Scale(SAS),the Self-rating Depression Scale(SDS) were filled out by both groups before and after practicing in ICU, and assessed the comprehensive ability after ICU practice. Results The scores of SAS and SDS after practicing were (49.47 ± 12.37),(50.25 ± 11.86)points in the observation group and (54.32 ± 12.51), (55.79 ± 11.34) points in the control group, and the difference was significant(t=10.822, 12.310, P<0.05). The total scores and personal quality, theoretical knowledge level, clinical nursing ability, judgment thinking ability, communication and cooperation ability was (88.45 ± 13.54), (23.21 ± 4.73), (18.42±3.45), (17.91±3.62), (8.56±0.83), (8.34±0.67) points in the observation group , and (73.13± 11.35), (19.13±4.52), (15.45±3.21), (15.76±3.54), (6.17±0.19), (5.17±0.18) points in the control group, and the difference was significant (t=3.226-8.665, P<0.05 or 0.01). Conlusions The narration education can reduce the psychological burden of baccalaureate nursing students practicing in ICU and can improve the comprehensive ability of them.